Review



csy4 cleavage sites  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc csy4 cleavage sites
    Csy4 Cleavage Sites, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/csy4 cleavage sites/product/Addgene inc
    Average 93 stars, based on 8 article reviews
    csy4 cleavage sites - by Bioz Stars, 2026-04
    93/100 stars

    Images



    Similar Products

    csy4 dr  (GenScript corporation)
    90
    GenScript corporation csy4 dr
    Csy4 Dr, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/csy4 dr/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    csy4 dr - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    csy4 cleavage sites  (Addgene inc)
    93
    Addgene inc csy4 cleavage sites
    Csy4 Cleavage Sites, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/csy4 cleavage sites/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    csy4 cleavage sites - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    monocot optimized cas9 and csy4 protein  (Addgene inc)
    90
    Addgene inc monocot optimized cas9 and csy4 protein
    Monocot Optimized Cas9 And Csy4 Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monocot optimized cas9 and csy4 protein/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    monocot optimized cas9 and csy4 protein - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    csy4 expression cassette  (GenScript corporation)
    90
    GenScript corporation csy4 expression cassette
    One‐step generation of multiple precise genome edits via the <t>Csy4‐mediated</t> processing system. A) Structure of the vectors of three‐pegRNAs containing cPE2 and epiPE2 and statistical analysis of the efficiencies of individual pegRNAs. B) Summary of mutation frequencies at three target sites by the three‐pegRNA epiPE2‐Csy4 system in HEK293T cells. C) Schematic representation of the genotyping results for all 48 cell colonies categorized as biallelic mutation, monoallelic mutation and wild type. D) Structure of the vector used to express pegRNAs 1 to 10 via the U6 promoter and efficiency analysis. E) Structure of the vector used to express pegRNAs 1 to 10 via the EF1α promoter and efficiency analysis.
    Csy4 Expression Cassette, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/csy4 expression cassette/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    csy4 expression cassette - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    pe2-u6-puro-csy4  (GenScript corporation)
    90
    GenScript corporation pe2-u6-puro-csy4
    One‐step generation of multiple precise genome edits via the <t>Csy4‐mediated</t> processing system. A) Structure of the vectors of three‐pegRNAs containing cPE2 and epiPE2 and statistical analysis of the efficiencies of individual pegRNAs. B) Summary of mutation frequencies at three target sites by the three‐pegRNA epiPE2‐Csy4 system in HEK293T cells. C) Schematic representation of the genotyping results for all 48 cell colonies categorized as biallelic mutation, monoallelic mutation and wild type. D) Structure of the vector used to express pegRNAs 1 to 10 via the U6 promoter and efficiency analysis. E) Structure of the vector used to express pegRNAs 1 to 10 via the EF1α promoter and efficiency analysis.
    Pe2 U6 Puro Csy4, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe2-u6-puro-csy4/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    pe2-u6-puro-csy4 - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    pemax  (Addgene inc)
    90
    Addgene inc pemax
    One‐step generation of multiple precise genome edits via the <t>Csy4‐mediated</t> processing system. A) Structure of the vectors of three‐pegRNAs containing cPE2 and epiPE2 and statistical analysis of the efficiencies of individual pegRNAs. B) Summary of mutation frequencies at three target sites by the three‐pegRNA epiPE2‐Csy4 system in HEK293T cells. C) Schematic representation of the genotyping results for all 48 cell colonies categorized as biallelic mutation, monoallelic mutation and wild type. D) Structure of the vector used to express pegRNAs 1 to 10 via the U6 promoter and efficiency analysis. E) Structure of the vector used to express pegRNAs 1 to 10 via the EF1α promoter and efficiency analysis.
    Pemax, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pemax/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    pemax - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    csy4  (Addgene inc)
    90
    Addgene inc csy4
    Fig. 3 | Expression of pegRNA with a POL II-promoter enhances prime editing delivery. a Structure of post-splicing ipegRNA and epegRNA.Created in BioRender. Ohlmann, T. (2024) https://BioRender.com/g43z718. b Efficacy of PE measured in SWYS cells transduced with GAG-PEV4-VLPs produced from cells expressing intronic-pegRNAs. Production was performed with addition of the <t>Csy4</t> protein or its non-cleaving mutant Csy4H29A. (n = 9) c Comparison of GAG-PEV4 and GAG-PE- V7 for the production of ipeg-loaded VLPs. (n = 9). d Impact of ipegRNA on VLP-
    Csy4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/csy4/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    csy4 - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    One‐step generation of multiple precise genome edits via the Csy4‐mediated processing system. A) Structure of the vectors of three‐pegRNAs containing cPE2 and epiPE2 and statistical analysis of the efficiencies of individual pegRNAs. B) Summary of mutation frequencies at three target sites by the three‐pegRNA epiPE2‐Csy4 system in HEK293T cells. C) Schematic representation of the genotyping results for all 48 cell colonies categorized as biallelic mutation, monoallelic mutation and wild type. D) Structure of the vector used to express pegRNAs 1 to 10 via the U6 promoter and efficiency analysis. E) Structure of the vector used to express pegRNAs 1 to 10 via the EF1α promoter and efficiency analysis.

    Journal: Advanced Science

    Article Title: Enhancing Prime Editing Efficiency Through Modulation of Methylation on the Newly Synthesized DNA Strand and Prolonged Expression

    doi: 10.1002/advs.202417790

    Figure Lengend Snippet: One‐step generation of multiple precise genome edits via the Csy4‐mediated processing system. A) Structure of the vectors of three‐pegRNAs containing cPE2 and epiPE2 and statistical analysis of the efficiencies of individual pegRNAs. B) Summary of mutation frequencies at three target sites by the three‐pegRNA epiPE2‐Csy4 system in HEK293T cells. C) Schematic representation of the genotyping results for all 48 cell colonies categorized as biallelic mutation, monoallelic mutation and wild type. D) Structure of the vector used to express pegRNAs 1 to 10 via the U6 promoter and efficiency analysis. E) Structure of the vector used to express pegRNAs 1 to 10 via the EF1α promoter and efficiency analysis.

    Article Snippet: To produce the epiPE2‐Csy4 vector, the Csy4 expression cassette was synthesized by blocking the AarI site (Genscript) and fused to epiPE2.

    Techniques: Mutagenesis, Plasmid Preparation

    Fig. 3 | Expression of pegRNA with a POL II-promoter enhances prime editing delivery. a Structure of post-splicing ipegRNA and epegRNA.Created in BioRender. Ohlmann, T. (2024) https://BioRender.com/g43z718. b Efficacy of PE measured in SWYS cells transduced with GAG-PEV4-VLPs produced from cells expressing intronic-pegRNAs. Production was performed with addition of the Csy4 protein or its non-cleaving mutant Csy4H29A. (n = 9) c Comparison of GAG-PEV4 and GAG-PE- V7 for the production of ipeg-loaded VLPs. (n = 9). d Impact of ipegRNA on VLP-

    Journal: Nature communications

    Article Title: Delivery of Prime editing in human stem cells using pseudoviral NanoScribes particles.

    doi: 10.1038/s41467-024-55604-0

    Figure Lengend Snippet: Fig. 3 | Expression of pegRNA with a POL II-promoter enhances prime editing delivery. a Structure of post-splicing ipegRNA and epegRNA.Created in BioRender. Ohlmann, T. (2024) https://BioRender.com/g43z718. b Efficacy of PE measured in SWYS cells transduced with GAG-PEV4-VLPs produced from cells expressing intronic-pegRNAs. Production was performed with addition of the Csy4 protein or its non-cleaving mutant Csy4H29A. (n = 9) c Comparison of GAG-PEV4 and GAG-PE- V7 for the production of ipeg-loaded VLPs. (n = 9). d Impact of ipegRNA on VLP-

    Article Snippet: Plasmids coding for PE2 (#132775), PEmax (#174820) and Csy4 (55196) were obtained from Addgene.

    Techniques: Expressing, Transduction, Produced, Mutagenesis, Comparison